ExFLP-22 Determination of Extraneous Material in bakery Products Without Fruit and Nut Ingredients
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F1BA9647511247A4A8F2EC8570ABE901 |
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0.08 |
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6 |
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日期： |
2012-3-3 |
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Published on the Food Directorate’s (Health Canada's) website at http://www.hc-sc.gc.ca/food-aliment,Government of Canada Gouvernement du Canada,Laboratory Procedure ExFLP-22,April 1997,HEALTH PRODUCTS AND FOOD BRANCH,OTTAWA,DETERMINATION OF EXTRANEOUS MATERIAL IN BAKERY PRODUCTS,WITHOUT FRUIT AND NUT INGREDIENTS,Bureau of Microbial Hazards,Food Directorate, Postal Locator: 2204A1,Health Canada, Ottawa, Ontario, K1A 0L2,1. APPLICATION,This method is applicable to the sampling and examination of bakery products without fruit and nut ingredients,for extraneous material, such as insect infestation, and rodent hairs to determine compliance with Sections 4, 5,and 7 of the Food and Drugs Act. This method replaces ExFLP-22 dated September 1987.,2. DEFINITION OF TERMS,A lot is defined as that amount (volume, weight, etc.) of the food which is produced, stored and/or shipped,under conditions as nearly uniform as possible, preferably designated by a common container code or marking,and, in any event, consisting of not more than one variety, grade or type of product from a single identifiable,source.,3. COLLECTION OF SAMPLES,3.1 Scrutinize the entire lot for live infestation. If live infestation is found, do not sample until after,fumigation or other effective treatment.,3.2 Obtain three sample units selected at random from the lot of at least 100 g each using appropriate,sampling equipment and containers. Three sample units constitute a sample.,3.3 Each sample unit must be kept separate and labelled 1, 2 and 3. Complete information respecting the,lot size, weight of individual containers, country of origin, exporter, importer, or domestic manufacturer,and product and lot identification should be recorded and should accompany the sample.,4. MATERIALS AND SPECIAL EQUIPMENT,1) Balance,2) 2-2.0 L beakers with covers (watch glasses),3) Autoclave. Either (a) slow exhaust type (Set slow exhaust to lower pressure from 1.15 to 0 kg per sq.,cm. in 15-20 min.) or (b) non-slow exhaust (Let cool to 0 kg per sq. cm. before opening or venting).,Alternatively instead of an autoclave use a steam bath.,4) Smooth magnetic stirring bar, teflon coated (1x5 cm),5) Stirrer hot plates,ExFLP-22,- 2 - April 1997,6) U.S. standard No. 230 sieve,7) 2 L percolator, premarked at 250 and 1700 mL levels,8) Rubber policeman attached to a long glass rod,9) Wash bottles,10) Suction filtration apparatus with Buchner or Hirsch funnel (5-7 cm perforated plate),11) Ruled filter paper. Filter paper should be larger than funnel plate (7-9 cm),12) Petri dishes to suit size of filter paper used,13) Stereoscopic microscope (10-30x),14) Ashless filter paper. Filter paper should be larger than funnel plate (7-9 cm),15) Drying oven (40-60oC),16) Crucible,17) Desiccator,18) Muffle oven,19) Emulsifiers, nonionic, water-soluble surfactants.,(1) Dialkylphenoxypoly (ethyleneoxy) ethanol - Igepal DM-710 (GAF Corporation).,(2) Nonylphenoxypoly (ethyleneoxy) ethanol - Igepal CO-730 (GAF Corporation).,20) Concentrated HCl,21) Antifoam solution. Antifoam "B" (BDH Chemicals),22) Mineral oil. Paraffin oil, white, light 125/135 Saybolt Universal Viscosity (38oC), specific gravity,0.840-0.860 (24oC),23) Alcohol, either 95% ethanol or 99% isopropanol,24) Detergent (1% sodium lauryl sulphate),25) Glycerol-alcohol (95%) mixture (1:1),26) Chloroform (CHCl3),5. PROCEDURE,The examination shall be carried out in accordance with the following instructions.,5.1 Preparation of Analytical Units,5.1.1 Thoroughly mix a sample unit and in a random fashion weigh 100 g. This 100 g constitutes an,analytical unit.,5.1.2 Repeat Step 5.1.1 for each of the remaining two sample units.,5.2 Isolation - Light Filth,5.2.1 Add 1 L hot (55-70oC) tap water to a 2 L beaker. Add 20 mL of emulsifier Igepal DM-710 and 5 mL of,emulsifier Igepal CO-730, and mix well. Add a 100 g analytical unit, breaking into pieces < 4 sq. cm.,ExFLP-22,- 3 - April 1997,Stir well. Proceed with digestion (a) or (b).,(a) Autoclave. Add 30 mL of HCl with stirring and 1 mL of antifoam solution. Autoclave for 30,min. at 121oC.,(b) Steam bath. Add 90 mL of HCl with stirring. Heat in a steam bath for 10 min. Add 1 mL of,antifoam solution. Boil for 15 min. on a magnetic stirrer-hot plate, keeping the beaker covered,with a watch glass.,5.2.2 Transfer slurry to a No. 230 sieve. Wash contents of sieve with a stream of hot (50-70oC) tap water,until washwater runs clear and the water runs freely through the material.,5.2.3 Transfer sieve retainings to original beaker with hot water (50-70oC) and dilute to approximately 1 L.,Add 30 mL of HCl and a magnetic stirring bar. Bring to a boil and boil 6 min.,5.2.4 A……
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